商品貨號:11765054
商品品牌:Gibco™
Description
Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
With |
• L-glutamine |
• Phenol Red |
The complete formulation is available.
Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture F-12 at two separate facilities; one located in Grand Island, NY and the other in Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
Specifications
Form: | Liquid |
---|---|
Glutamine: | L-Glutamine |
Green Features: | Sustainable packaging |
HEPES Buffer: | No HEPES |
Phenol Red Indicator: | Phenol Red |
Product Line: | Gibco® |
Product Size: | 500 mL |
Shipping Condition: | Room Temperature |
Sodium Pyruvate Additive: | Sodium Pyruvate |
Volume (Metric): | 500 ml |
Contents & storage
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture